Mechanistic Insights into the Metal-Dependent Activation of Zn^II-Dependent Metallochaperones.

ABSTRACT: Members of the COG0523 subfamily of candidate GTPase metallochaperones function in bacterial transition-metal homeostasis, but the nature of the cognate metal, mechanism of metal transfer, and identification of target protein(s) for metal delivery remain open questions. Here, we explore the multifunctionality of members of the subfamily linked to delivering Zn^II to apoprotein targets under conditions of host-imposed transition-metal depletion. We examine two zinc-uptake repressor (Zur)-regulated COG0523 family members, each from a major human pathogen, Acinetobacter baumannii (AbZigA) and Staphylococcus aureus (SaZigA), in an effort to develop a model for Zn^II metallochaperone activity. Zn^II chelator competition experiments revea

SUBMITTER: Jordan MR 

PROVIDER: S-EPMC7005846 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature