Self-Assembly of Lipopeptides Containing Short Peptide Fragments Derived from the Gastrointestinal Hormone PYY_3-36: From Micelles to Amyloid Fibrils.

ABSTRACT: We investigate the impact of lipidation on the self-assembly of two peptide fragments from the gastrointestinal peptide hormone PYY_3-36. The lipopeptides C₁₆IKPEAP and C₁₆IKPEAPGE contain the first 6 and 8 amino acid residues, respectively, from the PYY_3-36 peptide sequence, with a palmitoyl C₁₆ tail attached at the N-terminus. These lipopeptides form spherical micelles in aqueous solution, above a critical micelle concentration (cmc), which is pH-dependent. Modeling of small-angle X-ray scattering data along with molecular dynamics simulations shows the formation of micelles with a hydrophobic interior and a well-hydrated exterior. The lipopeptides have a disordered conformation over the pH and temperature ranges studied. The cmc is f

SUBMITTER: Hutchinson JA 

PROVIDER: S-EPMC7005981 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature