Long-term ex vivo haematopoietic-stem-cell expansion allows nonconditioned transplantation.

ABSTRACT: Multipotent self-renewing haematopoietic stem cells (HSCs) regenerate the adult blood system after transplantation¹, which is a curative therapy for numerous diseases including immunodeficiencies and leukaemias². Although substantial effort has been applied to identifying HSC maintenance factors through the characterization of the in vivo bone-marrow HSC microenvironment or niche^3-5, stable ex vivo HSC expansion has previously been unattainable^6,7. Here we describe the development of a defined, albumin-free culture system that supports the long-term ex vivo expansion of functional mouse HSCs. We used a systematic optimization approach, and found that high levels of thrombopoietin synergize with low levels of stem-cell factor and fibronectin to su

SUBMITTER: Wilkinson AC 

PROVIDER: S-EPMC7006049 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature