High copy number and highly stable Escherichia coli-Bacillus subtilis shuttle plasmids based on pWB980.

ABSTRACT:

Background

pWB980 derived from pUB110 is a promising expression vector in Bacillus for its high copy number and high stability. However, the low transformation rate of recombinant plasmids to the wild cells limited the application of it. On the basis of pWB980, constructing an E. coli-B. subtilis shuttle plasmid could facilitate the transformation rate to Bacillus cells. Because the insertion site for E. coli replication origin sequence (ori) is not unique in pWB980, in order to investigate the best insertion site, eight shuttle plasmids (pUC980-1 ~ pUC980-8) containing all possible insertion sites and directions were constructed.

Results

The results showed that all the selected insertion sites could be used to construct shuttle plasmid but some sites required a specific dir

SUBMITTER: Zhao X 

PROVIDER: S-EPMC7006159 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature