Molecular Signatures of Neuroinflammation Induced by αSynuclein Aggregates in Microglial Cells.

ABSTRACT: Alpha-synuclein (αSyn_Agg) are pathological hallmarks of Parkinson's disease (PD) and other synucleinopathies that induce microglial activation and immune-mediated neurotoxicity, but the molecular mechanisms of αSyn_Agg-induced immune activation are poorly defined. We performed quantitative proteomics by mass spectrometry coupled with PCR, immunohistochemical and functional validations studies to define the molecular characteristics of alpha synuclein mediated microglial activation. In mouse microglia, αSyn_Agg induced robust pro-inflammatory activation (increased expression of 864 genes including Irg1, Ifit1, and Pyhin) and increased nuclear proteins involved in RNA synthesis, splicing, and anti-viral defense mechanisms. Conversely, αSyn_Agg

SUBMITTER: Sarkar S 

PROVIDER: S-EPMC7006296 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature