Combined intramuscular and intraspinal transplant of bone marrow cells improves neuromuscular function in the SOD1^G93A mice.

ABSTRACT:

Background

The simultaneous contribution of several etiopathogenic disturbances makes amyotrophic lateral sclerosis (ALS) a fatal and challenging disease. Here, we studied two different cell therapy protocols to protect both central and peripheral nervous system in a murine model of ALS.

Methods

Since ALS begins with a distal axonopathy, in a first assay, we performed injection of bone marrow cells into two hindlimb muscles of transgenic SOD1^G93A mice. In a second study, we combined intramuscular and intraspinal injection of bone marrow cells. Fluorescence-activated cell sorting was used to assess the survival of the transplanted cells into the injected tissues. The mice were assessed from 8 to 16 weeks of age by means of locomotion and electrophysiological tests.

SUBMITTER: Martinez-Muriana A 

PROVIDER: S-EPMC7006400 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature