Serological diagnosis of Toxoplasma gondii: analysis of false-positive IgG results and implications.

ABSTRACT:

Background

Primary infection by Toxoplasma gondii in pregnant women can result in serious outcomes for the foetus. A false-positive IgG result during pregnancy can lead to a misdiagnosis of past infection and to stopping preventive measures. We collected 189 sera with positive Architect^® Toxo IgG assay (Abbott Laboratories) and negative IgG results with at least two other serological tests, in order to find an explanation for the suspected false-positive IgG results. We used the recomLine Toxoplasma IgG^® immunoblot (Mikrogen Diagnostik) to search for specific antigenic reactivities of the sera, and the LDBio Toxo II IgG^® immunoblot (LDBio Diagnostics) as a confirmatory test.

Results

The bands GRA8 and/or GRA7 were positive for 148 samples (78

SUBMITTER: Simon L 

PROVIDER: S-EPMC7006501 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature