Rational design of a DNA sequence-specific modular protein tag by tuning the alkylation kinetics.

ABSTRACT: Sequence-selective chemical modification of DNA by synthetic ligands has been a long-standing challenge in the field of chemistry. Even when the ligand consists of a sequence-specific DNA binding domain and reactive group, sequence-selective reactions by these ligands are often accompanied by off-target reactions. A basic principle to design DNA modifiers that react at specific sites exclusively governed by DNA sequence recognition remains to be established. We have previously reported selective DNA modification by a self-ligating protein tag conjugated with a DNA-binding domain, termed as a modular adaptor, and orthogonal application of modular adaptors by relying on the chemoselectivity of the protein tag. The sequence-specific crosslinking reaction by the modular adaptor is thought to p

SUBMITTER: Nguyen TM 

PROVIDER: S-EPMC7006624 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature