Integrated automated particle tracking microfluidic enables high-throughput cell deformability cytometry for red cell disorders.

ABSTRACT: Investigating individual red blood cells (RBCs) is critical to understanding hematologic diseases, as pathology often originates at the single-cell level. Many RBC disorders manifest in altered biophysical properties, such as deformability of RBCs. Due to limitations in current biophysical assays, there exists a need for high-throughput analysis of RBC deformability with single-cell resolution. To that end, we present a method that pairs a simple in vitro artificial microvasculature network system with an innovative MATLAB-based automated particle tracking program, allowing for high-throughput, single-cell deformability index (sDI) measurements of entire RBC populations. We apply our technology to quantify the sDI of RBCs from healthy volunteers, Sickle cell disease (SCD) patients, a trans

SUBMITTER: Guruprasad P 

PROVIDER: S-EPMC7007699 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature