Integrative proteomics reveals an increase in non-degradative ubiquitylation in activated CD4⁺ T cells.

ABSTRACT: Despite gathering evidence that ubiquitylation can direct non-degradative outcomes, most investigations of ubiquitylation in T cells have focused on degradation. Here, we integrated proteomic and transcriptomic datasets from primary mouse CD4⁺ T cells to establish a framework for predicting degradative or non-degradative outcomes of ubiquitylation. Di-glycine remnant profiling was used to reveal ubiquitylated proteins, which in combination with whole-cell proteomic and transcriptomic data allowed prediction of protein degradation. Analysis of ubiquitylated proteins identified by di-glycine remnant profiling indicated that activation of CD4⁺ T cells led to an increase in non-degradative ubiquitylation. This correlated with an increase in non-proteasome-targeted K29, K3

SUBMITTER: Dybas JM 

PROVIDER: S-EPMC7007700 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature