Curcumin Has Anti-Proliferative and Pro-Apoptotic Effects on Tongue Cancer in vitro: A Study with Bioinformatics Analysis and in vitro Experiments.

ABSTRACT:

Purpose

This study focused on the mechanism underlying the therapeutic effect of curcumin against tongue cancer (TC).

Methods

Target genes of TC and curcumin were identified, respectively. Three datasets of TC from Gene Expression Omnibus were included, and then the differentially expressed genes were collected. After combing the data from The Cancer Genome Atlas, bioinformatics analyses were performed to investigate hub genes in terms of the functions and correlations. The proliferation and migration of TC cells were evaluated with CCK-8 assay and scratch wound healing assay, respectively. Cell apoptosis was evaluated by TUNEL assay, flow cytometry and Western blot. Cell cycle was determined by flow cytometry.

Results

In this study, 15 hub genes were identified (TK1,

SUBMITTER: Ma C 

PROVIDER: S-EPMC7007779 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature