Chaperone-Assisted Host-Guest Interactions Revealed by Single-Molecule Force Spectroscopy.

ABSTRACT: The recent discovery of ultra-high binding affinities in cucurbit[7]uril (CB7)-based host-guest pairs in an aqueous environment has rendered CB7 a rather attractive material in analytical and biomedical applications. Due to the lack of a molecular platform that can follow the same host-guest complex during repetitive mechanical measurements, however, mechanical stabilities of the CB7 system have not been revealed, hindering its potential to rival widely used conjugation pairs, such as streptavidin-biotin. Here, we assembled a DNA template in which a flexible DNA linker was exploited to keep the host (CB7) and guest (adamantane) in proximity. This platform not only increased the efficiency of the single-molecule characterization in optical tweezers but also clearly revealed mechanical featu

SUBMITTER: Pandey S 

PROVIDER: S-EPMC7007810 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature