Sex-specific Estrogen Levels and Reference Intervals from Infancy to Late Adulthood Determined by LC-MS/MS.

ABSTRACT:

Context

The lack of sensitive and robust analytical methods has hindered the reliable quantification of estrogen metabolites in subjects with low concentrations.

Objective

To establish sex-specific reference ranges for estrone (E1) and estradiol (E2) throughout life and to evaluate sex-differences using the state-of-the-art liquid chromatography tandem mass spectrometry (LC-MS/MS) method for quantification of E1, E2, and estriol (E3).

Design

LC-MS/MS method development and construction of estrogen reference ranges.

Settings

Population-based cross-sectional cohorts from the greater Copenhagen and Aarhus areas.

Participants

Healthy participants aged 3 months to 61 years (n = 1838).

Results

An isotope diluted LC-MS/MS method was developed and validat

SUBMITTER: Frederiksen H 

PROVIDER: S-EPMC7007877 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature