The industrial yeast Pichia pastoris is converted from a heterotroph into an autotroph capable of growth on CO₂.

ABSTRACT: The methylotrophic yeast Pichia pastoris is widely used in the manufacture of industrial enzymes and pharmaceuticals. Like most biotechnological production hosts, P. pastoris is heterotrophic and grows on organic feedstocks that have competing uses in the production of food and animal feed. In a step toward more sustainable industrial processes, we describe the conversion of P. pastoris into an autotroph that grows on CO₂. By addition of eight heterologous genes and deletion of three native genes, we engineer the peroxisomal methanol-assimilation pathway of P. pastoris into a CO₂-fixation pathway resembling the Calvin-Benson-Bassham cycle, the predominant natural CO₂-fixation pathway. The resulting strain can grow continuously with CO₂ as a sole

SUBMITTER: Gassler T 

PROVIDER: S-EPMC7008030 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature