Codon optimized membrane cofactor protein expression in α 1, 3 galactosyltransferase knockout pig cells improve protection against cytotoxicity of monkey serum.

ABSTRACT: In this study, we attempted to upgrade GT ^-MCP/-MCP pig genetically to express MCP at a higher level and additionally thrombomodulin (TBM), which have respective roles as a complement regulatory protein and a coagulation inhibitor. We constructed a dicistronic cassette consisting of codon-optimized MCP (mMCP) and TBM (m-pI2), designed for ubiquitous expression of MCP and endothelium specific expression of TBM. The cassette was confirmed to allow extremely increased MCP expression compared with non-modified MCP, and an endothelial-specific TBM expression. We thus transfected m-pI2 into ear-skin fibroblasts isolated from a GT ^-MCP/-MCP pig. By twice selection using magnetically activated cell sorting

SUBMITTER: Lee H 

PROVIDER: S-EPMC7008109 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature