Detection of polymorphism within leptin gene in Egyptian river buffalo and predict its effects on different molecular levels.

ABSTRACT:

Background

Leptin (LEP) regulates the glucose homeostasis directly and centrally by the regulation of the insulin levels or indirectly by alternation of the levels of the other glucose metabolism regulator hormones. The present investigation studied the polymorphism in LEP gene which is related to fertility in 81 female Egyptian river buffalo.

Results

The PCR-RFLP pattern of the gene using the restriction enzyme Eco91I showed that all the animals had monomorphic pattern in the studied gene which consists of CC. A 511-bp fragment from LEP gene was amplified and sequenced. The homology between the amplified LEP gene fragment in buffalo and cattle, sheep, goat, human, and mouse on the nucleotides sequence level was 99, 97, 97, 87, and 79%, respectively, and on the translated am

SUBMITTER: Mahrous KF 

PROVIDER: S-EPMC7008111 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature