Malonate degradation in Acinetobacter baylyi ADP1: operon organization and regulation by MdcR.

ABSTRACT: Transcriptional regulators in the LysR or GntR families are typically encoded in the genomic neighbourhood of bacterial genes for malonate degradation. While these arrangements have been evaluated using bioinformatics methods, experimental studies demonstrating co-transcription of predicted operons were lacking. Here, transcriptional regulation was characterized for a cluster of mdc genes that enable a soil bacterium, Acinetobacter baylyi ADP1, to use malonate as a carbon source. Despite previous assumptions that the mdc-gene set forms one operon, our studies revealed distinct promoters in two different regions of a nine-gene cluster. Furthermore, a single promoter is insufficient to account for transcription of mdcR, a regulatory gene that is convergent to othe

SUBMITTER: Stoudenmire JL 

PROVIDER: S-EPMC7008218 | biostudies-literature | 2017 May

REPOSITORIES: biostudies-literature