Identification of Protein Expression Changes in Hepatocellular Carcinoma through iTRAQ.

ABSTRACT:

Background

Hepatocellular carcinoma (HCC) is a malignant tumor associated with a poor prognosis. Serum biomarkers of HCC have the potential to improve the diagnosis, provide a means to monitor the tumors, and predict their malignancy. Proteins that are expressed differentially between HCC patients and normal controls have the potential to be biomarkers.

Method

Serum samples from 10 confirmed HCC patients and 10 controls were collected. The differentially expressed proteins in the serum were identified using an isobaric tags for relative and absolute quantitation- (iTRAQ-) based method. Potential serum biomarkers were validated by ELISA in another 20 HCC patients and 20 controls. Their expression data in HCC were extracted from The Cancer Genome Atlas (TCGA) dataset.

Resul

SUBMITTER: Zhang Y 

PROVIDER: S-EPMC7008262 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature