Serum Exosomal MicroRNAs as Potential Circulating Biomarkers for Endometriosis.

ABSTRACT:

Background

A reliable noninvasive biomarker is not yet available for endometriosis diagnosis. Novel biomarkers for the diagnosis of endometriosis are urgently needed. The molecular constituents of exosomes, especially exosomal microRNAs (miRNAs), have considerable potential as novel biomarkers for clinical diagnosis. This study is aimed at exploring aberrant exosomal miRNA profiles by using miRNA microarray and at providing more accurate molecular biomarkers of endometriosis.

Methods

Exosomes were isolated from the serum of patients with endometriosis and negative controls and identified by electron microscopy, nanoparticle tracking analysis, and Western blot. Exosomal miRNAs were profiled by miRNA microarrays. The expression of selective serum exosomal miRNA was validated b

SUBMITTER: Zhang L 

PROVIDER: S-EPMC7008302 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature