Metabolic imaging and secondary ion mass spectrometry to define the structure and function of liver with acute and chronic pathology.

ABSTRACT: Conventional techniques are insufficient precisely to describe the internal structure, the heterogeneous cell populations, and the dynamics of biological processes occurring in diseased liver during surgery. There is a need for a rapid and safe method for the successful diagnosis of liver disease in order to plan surgery and to help avoid postoperative liver failure. We analyze the progression of both acute (cholestasis) and chronic (fibrosis) liver pathology using multiphoton microscopy with fluorescence lifetime imaging and second-harmonic generation modes combined with time-of-flight secondary ion mass spectrometry chemical analysis to obtain new data about pathological changes to hepatocytes at the cellular and molecular levels. All of these techniques allow the study of cellular metab

SUBMITTER: Kuznetsova D 

PROVIDER: S-EPMC7008498 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature