Ontology highlight
ABSTRACT:
SUBMITTER: Scheres SHW
PROVIDER: S-EPMC7008511 | biostudies-literature | 2020 Feb
REPOSITORIES: biostudies-literature
Acta crystallographica. Section D, Structural biology 20200130 Pt 2
Helical reconstruction in RELION is increasingly being used to determine the atomic structures of amyloid filaments from electron cryo-microscopy (cryo-EM) images. However, because the energy landscape of amyloid refinements is typically fraught with local optima, amyloid structure determination is often difficult. This paper aims to help RELION users in this process. It discusses aspects of helical reconstruction that are particularly relevant to amyloids, it illustrates the problem of local op ...[more]