Autophagy is critical for group 2 innate lymphoid cell metabolic homeostasis and effector function.

ABSTRACT:

Background

Allergic asthma is a chronic inflammatory disorder characterized by airway hyperreactivity (AHR) and driven by T_H2 cytokine production. Group 2 innate lymphoid cells (ILC2s) secrete high amounts of T_H2 cytokines and contribute to the development of AHR. Autophagy is a cellular degradation pathway that recycles cytoplasmic content. However, the role of autophagy in ILC2s remains to be fully elucidated.

Objective

We characterized the effects of autophagy deficiency on ILC2 effector functions and metabolic balance.

Methods

ILC2s from autophagy-deficient mice were isolated to evaluate proliferation, apoptosis, cytokine secretion, gene expression and cell metabolism. Also, autophagy-deficient ILC2s were adoptively transferred into Rag^-

SUBMITTER: Galle-Treger L 

PROVIDER: S-EPMC7010567 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature