Enhanced differentiation of human dopaminergic neuronal cell model for preclinical translational research in Parkinson's disease.

ABSTRACT: Human-derived neuronal cell lines are progressively being utilized in understanding neurobiology and preclinical translational research as they are biologically more relevant than rodent-derived cells lines. The Lund human mesencephalic (LUHMES) cell line comprises human neuronal cells that can be differentiated to post-mitotic neurons and is increasingly being used as an in vitro model for various neurodegenerative diseases. A previously published 2-step differentiation procedure leads to the generation of post-mitotic neurons within 5-days, but only a small proportion (10%) of the total cell population tests positive for tyrosine hydroxylase (TH). Here we report on a novel differentiation protocol that we optimized by using a cocktail of neurotrophic factors, pleiotropic cytokines, and a

SUBMITTER: Harischandra DS 

PROVIDER: S-EPMC7010568 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature