Elucidation of protein biomarkers for verification of selected biological warfare agents using tandem mass spectrometry.

ABSTRACT: Some pathogens and toxins have the potential to be used as weapons of mass destruction and instigate population-based fear. Efforts to mitigate biothreat require development of efficient countermeasures which in turn relies on fast and accurate methods to detect the biological agents in a range of complex matrices including environmental and clinical samples. We report here an mass spectrometry (MS) based methodology, employing both targeted and shot-gun approaches for the verification of biological agents from the environmental samples. Our shot-gun methodology relied on tandem MS analysis of abundant peptides from the spiked samples, whereas, the targeted method was based on an extensive elucidation of marker proteins and unique peptides resulting in the generation of an inclusion list o

SUBMITTER: Rajoria S 

PROVIDER: S-EPMC7010682 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature