Active nuclear import of the deacetylase Sirtuin-2 is controlled by its C-terminus and importins.

ABSTRACT: The NAD-dependent deacetylase Sirtuin-2 (SIRT2) functions in diverse cellular processes including the cell cycle, metabolism, and has important roles in tumorigenesis and bacterial infection. SIRT2 predominantly resides in the cytoplasm but can also function in the nucleus. Consequently, SIRT2 localisation and its interacting partners may greatly impact its function and need to be defined more clearly. In this study we used mass spectrometry to determine the interactomes of SIRT2 in whole cells and in specific cellular fractions; cytoplasm, nucleus and chromatin. Using this approach, we identified novel interacting partners of SIRT2. These included a number of proteins that function in nuclear import. We show that multiple importins interact with and contribute to the basal nuclear shuttli

SUBMITTER: Eldridge MJG 

PROVIDER: S-EPMC7010746 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature