Association between variants of the autophagy related gene ATG16L1 in inflammatory bowel diseases and clinical statues.

ABSTRACT:

Aim

In the present study, two main variants of ATG16L1 gene, rs2241880 T300A and rs2241879 C/T, were evaluated in IBD patients as well as in remission and flareup phase across an Iranian population for the first time.

Background

Inflammatory bowel disease (IBD) has found increasing global incidence and prevalence in recent years especially among pediatrics. ATG16L1 is the major gene that regulates autophagy pathway. The autophagy pathway also affects dysbiosis.

Methods

Genomic DNA was isolated from peripheral blood samples following salting out extraction method. The genotypes of ATG16L1 polymorphisms rs2241880 T300A and rs2241879 C/T were determined using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method.

Results

In this case

SUBMITTER: Baradaran Ghavami S 

PROVIDER: S-EPMC7011055 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature