Paracrine signals of mesenchymal stem cells induce epithelial to mesenchymal transition in gastric cancer cells.

ABSTRACT:

Aim

Given the high similarity of phenotypical and secretory properties of mesenchymal stem cells and fibroblasts, this study investigated the possibility of inducing EMT process by mesenchymal stem cells.

Background

Annually, more than 13% of deaths worldwide occur due to cancer. One of the main reasons for the high mortality rate is due to the metastasis of cancer stem cells. Induction of metastasis occurs during the EMT process, which can also be stimulated by fibroblast cells.

Methods

Mesenchymal stem cells (MSCs) were isolated and sub-cultured until passage 3 or 4. AGS cells were co-cultured with MSCs for 4 days. As the positive control group, AGS cells were treated with TGF-β (10ng/ml) for 48h. Finally, the mRNA expression level of Vimentin, β-catenin, Snail, and

SUBMITTER: Azizi P 

PROVIDER: S-EPMC7011073 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature