A post-transcriptional program of chemoresistance by AU-rich elements and TTP in quiescent leukemic cells.

ABSTRACT:

Background

Quiescence (G0) is a transient, cell cycle-arrested state. By entering G0, cancer cells survive unfavorable conditions such as chemotherapy and cause relapse. While G0 cells have been studied at the transcriptome level, how post-transcriptional regulation contributes to their chemoresistance remains unknown.

Results

We induce chemoresistant and G0 leukemic cells by serum starvation or chemotherapy treatment. To study post-transcriptional regulation in G0 leukemic cells, we systematically analyzed their transcriptome, translatome, and proteome. We find that our resistant G0 cells recapitulate gene expression profiles of in vivo chemoresistant leukemic and G0 models. In G0 cells, canonical translation initiation is inhibited; yet we find that inflammatory genes are

SUBMITTER: Lee S 

PROVIDER: S-EPMC7011231 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature