Effect of the TetR family transcriptional regulator Sp1418 on the global metabolic network of Saccharopolyspora pogona.

ABSTRACT:

Background

Saccharopolyspora pogona is a prominent industrial strain due to its production of butenyl-spinosyn, a high-quality insecticide against a broad spectrum of insect pests. TetR family proteins are diverse in a tremendous number of microorganisms and some are been researched to have a key role in metabolic regulation. However, specific functions of TetR family proteins in S. pogona are yet to characterize.

Results

In the present study, the overexpression of the tetR-like gene sp1418 in S. pogona resulted in marked effects on vegetative growth, sporulation, butenyl-spinosyn biosynthesis, and oxidative stress. By using qRT-PCR analysis, mass spectrometry, enzyme activity detection, and sp1418 knockout verification, we showed that most of these effects could be attribut

SUBMITTER: He H 

PROVIDER: S-EPMC7011500 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature