Mammalian gene expression variability is explained by underlying cell state.

ABSTRACT: Gene expression variability in mammalian systems plays an important role in physiological and pathophysiological conditions. This variability can come from differential regulation related to cell state (extrinsic) and allele-specific transcriptional bursting (intrinsic). Yet, the relative contribution of these two distinct sources is unknown. Here, we exploit the qualitative difference in the patterns of covariance between these two sources to quantify their relative contributions to expression variance in mammalian cells. Using multiplexed error robust RNA fluorescent in situ hybridization (MERFISH), we measured the multivariate gene expression distribution of 150 genes related to Ca²⁺ signaling coupled with the dynamic Ca²⁺ response of live cells to ATP. We show tha

SUBMITTER: Foreman R 

PROVIDER: S-EPMC7011657 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature