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Identification of anti-CD16a single domain antibodies and their application in bispecific antibodies.

ABSTRACT: CD16a (FcγRIIIa) mediates the antibody dependent cellular cytotoxicity (ADCC) and is important for anti-tumor activities of many therapeutic antibodies. Bispecific antibody targeting natural killer (NK) cells has been studied for cancer therapy. In this work, anti-CD16a single-domain antibodies were identified from hCD16a immunized camel. Bispecific antibodies are then constructed by fusing these single domain antibodies with an anti-CEA single domain antibody. These bispecific antibodies can recruite NK cells to kill CEA-positive tumor cells, and inhibit tumor growth in vivo, suggesting that these anti-CD16a single domain antibodies are powerful tools to engaging NK cells for cancer therapy.

SUBMITTER: Zhao Y

PROVIDER: S-EPMC7012161 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature

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Identification of anti-CD16a single domain antibodies and their application in bispecific antibodies.

Zhao Yining Y Li Yumei Y Wu Xiaoqiong X Li Li L Liu Jiayu J Wang Yanlan Y Liu Yue Y Li Qing Q Wang Zhong Z

Cancer biology & therapy 20190929 1

CD16a (FcγRIIIa) mediates the antibody dependent cellular cytotoxicity (ADCC) and is important for anti-tumor activities of many therapeutic antibodies. Bispecific antibody targeting natural killer (NK) cells has been studied for cancer therapy. In this work, anti-CD16a single-domain antibodies were identified from hCD16a immunized camel. Bispecific antibodies are then constructed by fusing these single domain antibodies with an anti-CEA single domain antibody. These bispecific antibodies can re ...[more]

PMID: 31564196

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