Brief Report: Complete Genome Sequence of CG-0018a-01 Establishes HIV-1 Subtype L.

ABSTRACT:

Background

The full spectrum of HIV-1 diversity can be found in Central Africa, including 2 divergent HIV-1 strains collected in 1983 and 1990 in Democratic Republic of Congo (DRC) that were preliminarily classified as group M subtype L. However, a third epidemiologically distinct subtype L genome must be identified to designate L as a true subtype.

Methods

Specimen CG-0018a-01 was collected in 2001 in DRC as part of an HIV diversity study. Previous subgenomic HIV-1 sequences from this specimen branched closely with proposed subtype L references. Metagenomic next-generation sequencing (mNGS) and HIV-specific target-enriched (HIV-xGen) libraries were combined for NGS to extend genome coverage. mNGS reads were analyzed for the presence of other coinfections with the sequence-b

SUBMITTER: Yamaguchi J 

PROVIDER: S-EPMC7012332 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature