Induction of RNA interference to block Zika virus replication and transmission in the mosquito Aedes aegypti.

ABSTRACT: The yellow fever mosquito, Aedes aegypti, serves as the primary vector for epidemic transmission of yellow fever, dengue, Zika (ZIKV), and chikungunya viruses to humans. Control of Ae. aegypti is currently limited to insecticide applications and larval habitat management; however, to combat growing challenges with insecticide resistance, novel genetic approaches for vector population reduction or transmission interruption are being aggressively pursued. The objectives of this study were to assess the ability of the Ae. aegypti antiviral exogenous-small interfering RNA (exo-siRNA) response to inhibit ZIKV infection and transmission, and to identify the optimal RNA interference (RNAi) target region in the ZIKV genome. We accomplished these objectives by in vitro transcription of five long do

SUBMITTER: Magalhaes T 

PROVIDER: S-EPMC7012353 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature