Imaging plant germline differentiation within Arabidopsis flowers by light sheet microscopy.

ABSTRACT: In higher plants, germline differentiation occurs during a relatively short period within developing flowers. Understanding of the mechanisms that govern germline differentiation lags behind other plant developmental processes. This is largely because the germline is restricted to relatively few cells buried deep within floral tissues, which makes them difficult to study. To overcome this limitation, we have developed a methodology for live imaging of the germ cell lineage within floral organs of Arabidopsis using light sheet fluorescence microscopy. We have established reporter lines, cultivation conditions, and imaging protocols for high-resolution microscopy of developing flowers continuously for up to several days. We used multiview imagining to reconstruct a three-dimensional model of

SUBMITTER: Valuchova S 

PROVIDER: S-EPMC7012603 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature