Cellular uptake of protic ruthenium complexes is influenced by pH dependent passive diffusion and energy dependent efflux.

ABSTRACT: The lipophilic vs. hydrophilic properties of three protic ruthenium compounds were studied as a function of pH. Specifically, we measured Log(D_o/w) values for [(N,N)₂Ru(6,6'-dhbp)]²⁺ complexes (where N,N = 2,2'-bipyridine (1_A), 1,10-phenanthroline (2_A), 2,3-dihydro-[1,4]dioxino[2,3-f][1,10]phenanthroline (3_A) and 6,6'-dhbp is the diprotic 6,6'-dihydroxy-2,2'-bipyridine ligand) from pH 4.0 to 8.0. This study allowed us to demonstrate that as the ligand is deprotonated at higher pH values the resulting neutral charge on the complex improves its lipophilic properties. Thus, improved uptake by passive diffusion is expected with protic ligands on Ru(II). Furthermore, cellular studies have demonstrated that passive diffusion is th

SUBMITTER: Park S 

PROVIDER: S-EPMC7012720 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature