Identification of novel microRNAs and their targets in Chlorophytum borivilianum by small RNA and degradome sequencing.

ABSTRACT: Plant specific miRNAs (Novel miRNAs) are well known to perform distinctive functions in biological processes. Identification of new miRNAs is necessary to understand their gene regulation. Degradome provides an opportunity to explore the miRNA functions by comparing the miRNA population and their degraded products. In the present study, Small RNA sequencing data was used to identify novel miRNAs. Further, degradome sequencing was carried out to identify miRNAs targets in the plant, Chlorophytum borivilianum. The present study supplemented 40 more novel miRNAs correlating degradome data with smallRNAome. Novel miRNAs, complementary to mRNA partial sequences obtained from degradome sequencing were actually targeting the later. A big pool of miRNA was established by using Oryza sati

SUBMITTER: Kajal M 

PROVIDER: S-EPMC7012778 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature