Remote biosensor for the determination of trypsin by using nanoporous anodic alumina as a three-dimensional nanostructured material.

ABSTRACT: The determination of trypsin in the human real sample is a routine medical investigation to assess the pancreatic disease. Herein, we fabricated an interferometric reflectance spectroscopy based biosensor for the determination trypsin. For this purpose, urease and fluorescein 5(6)-isothiocyanate (FLITC) were immobilized on the nanoporous anodic alumina (NAA). The operation principle of the proposed biosensor is based on the change in the pH of the solution during the reaction of urease and urea and therefore change in the light-absorbing ability of FLITC in the presence of trypsin. The reaction of the urease enzyme with urea increased the pH of the solution because of producing ammonia. This increase in the pH of solution increased the light-absorbing ability of the immobilized FLITC on NA

SUBMITTER: Amouzadeh Tabrizi M 

PROVIDER: S-EPMC7012875 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature