Histamine up-regulates oncostatin M expression in human M1 macrophages.

ABSTRACT:

Background and purpose

Human monocyte-derived M1 macrophages develop in relation to growth factors, bacterial products, and cytokines in a local micro-environment. M1 macrophages produce pro-inflammatory mediators, in particular, oncostatin M (OSM), which is secreted from the cells in response to the active complement component C5a. As C5a also releases histamine from human mast cells and shows immune modulatory functions similar to histamine in regulating expression of the IL-12 cytokine family, we investigated the effects of histamine on OSM expression in human M1 macrophages.

Experimental approach

Cytokine expression was analysed by real-time quantitative PCR and elisa techniques. Normal human epidermal keratinocytes were stimulated with supernatants from activated M1 mac

SUBMITTER: Mommert S 

PROVIDER: S-EPMC7012943 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature