Stimulation of histamine H₄ receptors increases the production of IL-9 in Th9 polarized cells.

ABSTRACT:

Background and purpose

Th9 cells represent a recently defined subset of CD4⁺ T-helper cells, characterized by a high production of IL-9. They are found at increased frequency in lesions of atopic dermatitis, where IL-9 is also elevated. As histamine is up-regulated in lesions of inflammatory skin diseases, we investigated the expression profile of histamine receptors and their functional role on Th9 cells.

Experimental approach

Naïve CD4⁺ T-cells were purified from human peripheral blood mononuclear cells, using magnetic beads and further differentiated into Th9 cells. During differentiation, cells were additionally stimulated with histamine receptor agonists or left untreated. Histamine receptor expression as well as IL-9 production was measured.

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SUBMITTER: Schaper-Gerhardt K 

PROVIDER: S-EPMC7012944 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature