Ontology highlight
ABSTRACT:
SUBMITTER: Kuo HH
PROVIDER: S-EPMC7013200 | biostudies-literature | 2020 Feb
REPOSITORIES: biostudies-literature
Stem cell reports 20200109 2
Human induced pluripotent stem cell (hiPSC) culture has become routine, yet the cost of pluripotent cell media, frequent medium changes, and the reproducibility of differentiation have remained restrictive. Here, we describe the formulation of a hiPSC culture medium (B8) as a result of the exhaustive optimization of medium constituents and concentrations, establishing the necessity and relative contributions of each component to the pluripotent state and cell proliferation. The reagents in B8 re ...[more]