Mechanisms of Tumor-Lymphatic Interactions in Invasive Breast and Prostate Carcinoma.

ABSTRACT: During the last few years, diverse studies have shown that tumors can actively interact with the lymphatic system and promote metastases development. In order to examine the molecular mechanisms involved in this interaction, we co-cultured tumor and lymphatic endothelial cells (LEC) and subsequently analyzed the molecular alterations of LECs. Therefore, LECs were co-cultivated with either a highly or weakly metastatic breast cancer cell line using contact (mixture) and non-contact (transwell) co-cultures. mRNA profiles from LECs were subsequently analyzed for genes specifically induced by highly metastatic tumor cells ("metastatic specific"). Among the up-regulated "metastatic specific" genes, we found candidates involved in cell cycle, cell adhesion and motility (BST2, E-selectin, and HMM

SUBMITTER: Oliveira-Ferrer L 

PROVIDER: S-EPMC7013901 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature