A custom-built low-cost chamber for exposing rodents to e-cigarette aerosol: practical considerations.

ABSTRACT: Objectives: To (1) design and build a low-cost exposure chamber system for whole-body exposure of rodents to electronic cigarette aerosol, (2) provide detailed instructions with particular focus on automated e-cigarette activation, and (3) develop a simple mathematical model for aerosol levels in the exposure chamber.Methods: We built the system with standard laboratory equipment and an open-source electronics platform (Arduino) for e-cigarette activation. Arduino is used to control a solenoid, which pushes the activation button of so-called "Mod" e-cigarettes, and a pump to move the aerosol from the mouthpiece of the e-cigarette into the chamber. For "Pods" and "Cigalikes," the solenoid is not used as they are activated by the vacuum created by the pump. Aerosol concentratio

SUBMITTER: Hilpert M 

PROVIDER: S-EPMC7013998 | biostudies-literature | 2019 Sep - Oct

REPOSITORIES: biostudies-literature