Ecotopic Expression of the Antimicrobial Peptide DmAMP1W Improves Resistance of Transgenic Wheat to Two Diseases: Sharp Eyespot and Common Root Rot.

ABSTRACT: Wheat (Triticum aestivum L.) is an important staple crop. Sharp eyespot and common root rot are destructive diseases of wheat. Antimicrobial peptides (AMPs) are small peptides with broad-spectrum antimicrobial activity. In this study, we synthesized the DmAMP1W gene, encoding Dahlia merckii DmAMP1, and investigated the antifungal role of DmAMP1W in vitro and in transgenic wheat. Protein electrophoresis analysis and in vitro inhibition results demonstrated that the synthesized DmAMP1W correctly translated to the expected peptide DmAMP1W, and the purified peptide inhibited growths of the fungi Rhizoctonia cerealis and Bipolaris sorokiniana, the pathogenic causes of wheat sharp eyespot and common root rot. DmAMP1W was introduced into a wheat variety Zhoumai18 via Agrobacterium-mediated transf

SUBMITTER: Su Q 

PROVIDER: S-EPMC7014311 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature