Molecular Profile of Phage Infection: A Novel Approach for the Characterization of Erwinia Phages through qPCR.

ABSTRACT: Due to the emergence of antibiotic resistance, phage-mediated biocontrol has become an attractive alternative for pathogen management in agriculture. While the infection characteristics of many phages can be adequately described using plaque assays and optical density, the results from phages of the apple pathogen Erwiniaamylovora have low reproducibility with these techniques. Using quantitative real-time PCR (qPCR), the stage of the lytic cycle was determined through a combination of chloroform-based sampling, centrifugation, and DNase treatment. Monitoring the transition of phage genomes through the lytic cycle generates a molecular profile from which phage infection characteristics such as adsorption rate and burst size can be determined. To our knowledge, this is the first report of q

SUBMITTER: Parcey M 

PROVIDER: S-EPMC7014438 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature