Accumulation of DNA methylation alterations in paediatric glioma stem cells following fractionated dose irradiation.

ABSTRACT:

Background

Radiation is an important therapeutic tool. However, radiotherapy has the potential to promote co-evolution of genetic and epigenetic changes that can drive tumour heterogeneity, formation of radioresistant cells and tumour relapse. There is a clinical need for a better understanding of DNA methylation alterations that may follow radiotherapy to be able to prevent the development of radiation-resistant cells.

Methods

We examined radiation-induced changes in DNA methylation profiles of paediatric glioma stem cells (GSCs) in vitro. Five GSC cultures were irradiated in vitro with repeated doses of 2 or 4 Gy. Radiation was given in 3 or 15 fractions. DNA methylation profiling using Illumina DNA methylation arrays was performed at 14 days post-radiation. The cellular c

SUBMITTER: Danielsson A 

PROVIDER: S-EPMC7014676 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature