Vitamin D repletion ameliorates adipose tissue browning and muscle wasting in infantile nephropathic cystinosis-associated cachexia.

ABSTRACT:

Background

Ctns^-/- mice, a mouse model of infantile nephropathic cystinosis, exhibit hypermetabolism with adipose tissue browning and profound muscle wasting. Ctns^-/- mice are 25(OH)D₃ and 1,25(OH)₂ D₃ insufficient. We investigated whether vitamin D repletion could ameliorate adipose tissue browning and muscle wasting in Ctns^-/- mice.

Methods

Twelve-month-old Ctns^-/- mice and wild-type controls were treated with 25(OH)D₃ and 1,25(OH)₂ D₃ (75 μg/kg/day and 60 ng/kg/day, respectively) or an ethylene glycol vehicle for 6 weeks. Serum chemistry and parameters of energy homeostasis were measured. We quantitated total fat mass and studied expression of molecules regulating

SUBMITTER: Cheung WW 

PROVIDER: S-EPMC7015252 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature