Effects of Aluminum on the Integrity of the Intestinal Epithelium: An in Vitro and in Vivo Study.

ABSTRACT: BACKGROUND:Aluminum (Al) is the most abundant and ubiquitous metal in the environment. The main route of human exposure to Al is through food and water intake. Although human exposure to Al is common, the influence of Al on the gastrointestinal tract remains poorly understood. OBJECTIVES:We aimed to further understand the toxic effect of Al and to elucidate the underlying cellular mechanisms in the intestinal barrier. METHODS:The human intestinal epithelial cell line HT-29 and C57BL6 mice were exposed to AlCl3 at 0-16 mM (1-24h) and 5-50mg/kg body weight (13 weeks), respectively. In cell culture experiments, intracellular oxidative stress, inflammatory protein and gene expression, and intestinal epithelial permeability were measured. In animal studies, histological examination, gene expres

SUBMITTER: Jeong CH 

PROVIDER: S-EPMC7015552 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature