Expression of RcHSP70, heat shock protein 70 gene from Chinese rose, enhances host resistance to abiotic stresses.

ABSTRACT: There exist differences in the heat tolerance of Chinese rose varieties, and high temperature in summer can lead to failure of blooming in non-heat-tolerant Chinese rose varieties. We cloned a heat shock protein 70 gene (designated RcHSP70) from heat-tolerant varieties of Chinese rose (Rosa hybrida L.) to elucidate the molecular mechanism of heat tolerance and improve the quality of Chinese rose. Degenerate primers were designed for RcHSP70 according to the 5'- and 3'-end sequences of HSP70 genes in apple and tea. RcHSP70 was cloned from heat-tolerant Chinese rose varieties after heat shock. The heat shock-induced expression patterns of RcHSP70 in different Chinese rose varieties were analyzed by RT-PCR. Following heat shock (38 °C/3 h), RcHSP70 was highly expressed in the heat-tolerant va

SUBMITTER: Jiang C 

PROVIDER: S-EPMC7015905 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature