In-cell NMR as a sensitive tool to monitor physiological condition of Escherichia coli.

ABSTRACT: The in-cell NMR technique offers significant insights into the structure and function of heterologous proteins in the physiological intracellular environment at an atomic resolution. Escherichia coli (E. coli) is one of the most widely used host cells for heterologous protein expression in structural biological studies as well as for in-cell NMR studies to investigate fundamental structural characteristics and the physiochemistry of certain proteins and their intermolecular interactions under physiological conditions. However, in many cases, it is not easy to obtain well-resolved in-cell NMR spectra because the detectability and resolution of these spectra are significantly influenced by intracellular factors such as nonspecific intermolecular interactions. In this study, we re-examined th

SUBMITTER: Sugiki T 

PROVIDER: S-EPMC7015911 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature