Two-dimensional NMR lineshape analysis of single, multiple, zero and double quantum correlation experiments.

ABSTRACT: NMR spectroscopy provides a powerful approach for the characterisation of chemical exchange and molecular interactions by analysis of series of experiments acquired over the course of a titration measurement. The appearance of NMR resonances undergoing chemical exchange depends on the frequency difference relative to the rate of exchange, and in the case of one-dimensional experiments chemical exchange regimes are well established and well known. However, two-dimensional experiments present additional complexity, as at least one additional frequency difference must be considered. Here we provide a systematic classification of chemical exchange regimes in two-dimensional NMR spectra. We highlight important differences between exchange in HSQC and HMQC experiments, that on a practical level

SUBMITTER: Waudby CA 

PROVIDER: S-EPMC7015968 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature